Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Muscle
Cell type
RH-4
NA
NA

Attributes by original data submitter

Sample

source_name
RH4
cell line
RH4
diagnosis
rhabdomyosarcoma
treatment
7 days treatment with 250nM ACBI1 (active) PROTAC compound (University of Dundee, Institute of Chemical and Structural Biology available at Boehringer Ingelheim (opnMe))
genotype
wild type
antibody
anti-BRG1, Abcam, ab110641

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After reverse crosslinking, ChIP DNA was purified using MinElute PCR Purification Kit (Qiagen). ChIPseq Library

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
30626546
Reads aligned (%)
89.1
Duplicates removed (%)
91.9
Number of peaks
383 (qval < 1E-05)

hg19

Number of total reads
30626546
Reads aligned (%)
88.4
Duplicates removed (%)
92.8
Number of peaks
409 (qval < 1E-05)

Base call quality data from DBCLS SRA